Strain Data Sheet

RBRC11278

Strain Information

Image
BRC No.RBRC11278
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Pkn2<tm1.1Hmuk>
Former Common namePKN2<flox/flox>
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strain
Appearance
Strain developmentestablished in 2009. back-crossed to C57BL/6N (Charles River) more than 10 times.
Strain descriptionExons 18 and 19 of PKN2, encoding the area around the activation loop of the catalytic domain of this enzyme, are flanked by two loxP sequences in the same orientation.
Colony maintenance
ReferencesGenes Cells, 22(2):220-236 (2017). doi: 10.1111/gtc.12470. Epub 2017 Jan 19. 28102564

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Pkn2protein kinase N23Pkn2<tm1.1Hmuk>targeted mutation 1.1, Hideyuki Mukai

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site3Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP3loxP

Ordering Information

供与核酸phage P1 loxP sites, yeast FRT (flippase recombination target) site, mouse Pkn2 genomic DNA
Research applicationCre/loxP system
FLP/frt system
提供条件条件を付加する。
研究成果の公表にあたって寄託者の指定する文献を引用する。Genes Cells. 2017 Feb;22(2):220-236. doi: 10.1111/gtc.12470. Epub 2017 Jan 19.
利用者は、電子メールで事前に寄託者の承諾を得る。
Depositor向井　秀幸（国立大学法人神戸大学）
Strain Status凍結精子のアイコン凍結精子
Strain Availability凍結精子より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data