Strain Data Sheet
RBRC11273
Strain Information | |
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| Image | |
| BRC No. | RBRC11273 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6.129P2-Mapk14<tm1(D316N)Moga> |
| Former Common name | p38alpha floxSem |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | EB3/5 ES cells derived from E14tg2aThe homo mice were embryonic lethal. After removing the gene cassette by the cre recombinase, the mice expressed p38alpha Mapk with D316N sevenmaker type mutation and home mice were viable. |
| Strain description | The p38alpha (Mapk 14) gene is disrupted by by the insertion of an IRES-β-geo fusion cassette flanked by loxP sites. Homo mice are embryonic lethal.If the cassette is removed by cre mediated recombination, this mouse can conditionally express p38alpha with D316N sevenmaker type mutation (a docking site mutation).A 3.5-kb cassette flanked by two loxP sites is inserted between exons 10 and 11 of p38alpha gene. At the same time exon 11 is replaced by one coding the D316N sevenmaker type mutation. The cassette is generated by modifying the dicistronic targeting construct, pGT1.8IresBgeo (Mountford et al., 1994), consisting of a splice acceptor site (SA) from mouse En-2, an internal ribosomal entry site (IRES) from encephalomyocarditis virus (EMCV), a neomycin phosphotransferase gene (neo), and the SV40 polyadenylation sequence. |
| Colony maintenance | |
| References | J. Neuroinflammation, 10:1. (2013). 23282009 Nat. Biotechnol., 25(2):233-7 (2007). 17220877 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter En2En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence)17En2 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Iresinternal ribosomal entry site (EMCV)17 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP17loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)17 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyA adenylation17 |
Ordering Information | |
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| Donor DNA | Bacteriophage P1 loxP sites, mouse En-2 splice acceptor site, EMCV IRES, E. coli neo, SV40 Poly adenylation sequence, Mouse Mapk14 genomic DNA (exon 11) |
| Research application | Cre/loxP system |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Biotechnol., 25(2):233-7 (2007). J. Neuroinflammation, 10:1. (2013). |
| Depositor | Masato Ogata (Mie University) |
| Strain Status |  Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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