Strain Data Sheet

RBRC11272

Strain Information

Image
BRC No.RBRC11272
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Mapk1<tm3(D319N)Moga>
Former Common nameERK2 floxSem
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentEB3/5 ES cells derived from E14tg2aThe homo mice were embryonic lethal. After removing the gene cassette by the cre recombinase, the mice expressed ERK2 Mapk with D319N sevenmaker type mutation.
Strain descriptionThe ERK2 (Mapk 1) gene of ERK2 floxSem mouse is disrupted by by the insertion of an IRES-β-geo fusion cassette flanked by loxP sites. Homo mice are embryonic lethal.If the cassette is removed by cre mediated recombination, this mouse can conditionally express ERK2 with D319N sevenmaker type mutation (a docking site mutation).A 3.5-kb cassette flanked by two loxP sites is inserted after exons 2 of ERK2 gene. At the same time exon 4 is replaced by a partial cDNA of ERK2 starting from exon 3 to the end of the polyadenylation signal sequence. This cDNA codes the D319N sevenmaker type mutation. The cassette is generated by modifying the dicistronic targeting construct, pGT1.8IresBgeo (Mountford et al., 1994), consisting of a splice acceptor site (SA) from mouse En-2, an internal ribosomal entry site (IRES) from encephalomyocarditis virus (EMCV), a neomycin phosphotransferase gene (neo), and the SV40 polyadenylation sequence.
Colony maintenance
ReferencesGenes Cells. 8(11):847-56 (2003). 14622137

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Mapk1mitogen-activated protein kinase 116Mapk1<tm3(D319N)Moga>targeted mutation 3, Masato OgataErk2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
En2En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence)16En2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Iresinternal ribosomal entry site (EMCV)16

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP16loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)16

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA adenylation16

Ordering Information

供与核酸Bacteriophage P1 loxP sites, mouse En-2 splice acceptor site, EMCV IRES, E. coli neo, SV40 Poly adenylation sequence, mouse Mapk1 cDNA, pA, mouse Mapk1 genomic DNA
Research applicationCre/loxP system
提供条件条件を付加する。
研究成果の公表にあたって寄託者の指定する文献を引用する。Genes Cells. 8(11):847-56 (2003).
Depositor緒方　正人（国立大学法人三重大学）
Strain Status凍結精子のアイコン凍結精子
Strain Availability凍結精子より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data