Strain Data Sheet
RBRC11272
Strain Information | |
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| Image | |
| BRC No. | RBRC11272 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6.129P2-Mapk1<tm3(D319N)Moga> |
| Former Common name | ERK2 floxSem |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | EB3/5 ES cells derived from E14tg2aThe homo mice were embryonic lethal. After removing the gene cassette by the cre recombinase, the mice expressed ERK2 Mapk with D319N sevenmaker type mutation. |
| Strain description | The ERK2 (Mapk 1) gene of ERK2 floxSem mouse is disrupted by by the insertion of an IRES-β-geo fusion cassette flanked by loxP sites. Homo mice are embryonic lethal.If the cassette is removed by cre mediated recombination, this mouse can conditionally express ERK2 with D319N sevenmaker type mutation (a docking site mutation).A 3.5-kb cassette flanked by two loxP sites is inserted after exons 2 of ERK2 gene. At the same time exon 4 is replaced by a partial cDNA of ERK2 starting from exon 3 to the end of the polyadenylation signal sequence. This cDNA codes the D319N sevenmaker type mutation. The cassette is generated by modifying the dicistronic targeting construct, pGT1.8IresBgeo (Mountford et al., 1994), consisting of a splice acceptor site (SA) from mouse En-2, an internal ribosomal entry site (IRES) from encephalomyocarditis virus (EMCV), a neomycin phosphotransferase gene (neo), and the SV40 polyadenylation sequence. |
| Colony maintenance | |
| References | Genes Cells. 8(11):847-56 (2003). 14622137 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter En2En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence)16En2 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Iresinternal ribosomal entry site (EMCV)16 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP16loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)16 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyA adenylation16 |
Ordering Information | |
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| Donor DNA | Bacteriophage P1 loxP sites, mouse En-2 splice acceptor site, EMCV IRES, E. coli neo, SV40 Poly adenylation sequence, mouse Mapk1 cDNA, pA, mouse Mapk1 genomic DNA |
| Research application | Cre/loxP system |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genes Cells. 8(11):847-56 (2003). |
| Depositor | Masato Ogata (Mie University) |
| Strain Status |  Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |