Strain Data Sheet

RBRC11264

Strain Information

Image
BRC No.RBRC11264
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameB6D2-Rasef<em1Osb>
Former Common nameRasef<-63153/wt>
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2020. Fertilized eggs derived from BDF1 × BDF1 were used to generate this line. Mixed genetic background.
Strain descriptionRasef knockout mouse generated by the CRISPR/Cas9. 63,153 bp deletion in the Rasef gene.
Colony maintenance
ReferencesAsian J. Androl., (2021) doi: 10.4103/aja.aja_63_21. 34290169

Health Report

Examination Date / Room / Rack2023/04/10Room:3-BRack:K
2023/01/10Room:3-BRack:K
2022/10/11Room:3-BRack:K
2022/07/11Room:3-BRack:K
2022/06/13Room:3-BRack:K

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
RasefRAS and EF hand domain containing4Rasef<em1Osb>endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University

Ordering Information

Donor DNAStreptococcus pyogenes crRNA, tracrRNA, Mouse a part of Rasef gene
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Asian J Androl. Jul 16.(2021) doi: 10.4103/aja.aja_63_21.RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasahito Ikawa (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data