Strain Information | |
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Image | |
BRC No. | RBRC11243 |
Type | CRISPR/Cas9 (Transgene) |
Species | Mus musculus |
Strain name | B6D2-Dcst2<em2Osb> Tg(CAG/Su9-DsRed2,Acr3-EGFP)RBGS002Osb |
Former Common name | Dcst2<-25/wt> |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Deposited by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2020. Fertilized eggs derived from BDF1 × BDF1 were used to generate this line. Mixed genetic background. Homozygous males are infertile. |
Strain description | Dcst2 mutant mouse generated by the CRISPR/Cas9. 25 bp deletion in the Dcst2 gene. This strain also has a transgene expressing GFP in sperm acrosome from acr3-EGFP and RFP in sperm mitochondoria from CAG/su9-DsRed2. |
Colony maintenance | |
References | Commun Biol., 5(1):332 (2022). doi: 10.1038/s42003-022-03289-w. 35393517 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Dcst2DC-STAMP domain containing 23Dcst2<em2Osb>endonuclease-mediated mutation 2, Research Institute for Microbial Diseases, Osaka University Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter DsRed2Red Fluorescent Protein (Discosoma sp.)UNDsRed2 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)UNEGFP |
Ordering Information | |
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Donor DNA | Streptococcus pyogenes crRNA, tracrRNA, Mouse a part of Dcst2 gene, chicken beta-actin promoter, rabbit beta-globin polyadenylation signal, CMV CMV IE enhancer, mouse proacrosin signal peptide, mouse acrosin N-ternimal peptide, jellyfish GFP cDNA, mouse acrosin promoter, mouse su9 cDNA, coral DsRed2 cDNA |
Research application | Fluorescent Proteins/lacZ System |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Commun Biol Apr 7;5(1):332.(2022) doi: 10.1038/s42003-022-03289-w.RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. |
Depositor | Masahito Ikawa (Osaka University) |
Strain Status | Frozen sperm |
Strain Availability | Recovery and QC required prior to distribution |
Additional Info. | Necessary documents for ordering: |
BRC mice in Publications |
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No Data |