Strain Data Sheet

RBRC11243

Strain Information

Image
BRC No.RBRC11243
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameB6D2-Dcst2<em2Osb> Tg(CAG/Su9-DsRed2,Acr3-EGFP)RBGS002Osb
Former Common nameDcst2<-25/wt>
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2020. Fertilized eggs derived from BDF1 × BDF1 were used to generate this line. Mixed genetic background. Homozygous males are infertile.
Strain descriptionDcst2 mutant mouse generated by the CRISPR/Cas9. 25 bp deletion in the Dcst2 gene. This strain also has a transgene expressing GFP in sperm acrosome from acr3-EGFP and RFP in sperm mitochondoria from CAG/su9-DsRed2.
Colony maintenance
ReferencesCommun Biol., 5(1):332 (2022). doi: 10.1038/s42003-022-03289-w. 35393517

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Dcst2DC-STAMP domain containing 23Dcst2<em2Osb>endonuclease-mediated mutation 2, Research Institute for Microbial Diseases, Osaka University

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
DsRed2Red Fluorescent Protein (Discosoma sp.)UNDsRed2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)UNEGFP

Ordering Information

Donor DNAStreptococcus pyogenes crRNA, tracrRNA, Mouse a part of Dcst2 gene, chicken beta-actin promoter, rabbit beta-globin polyadenylation signal, CMV CMV IE enhancer, mouse proacrosin signal peptide, mouse acrosin N-ternimal peptide, jellyfish GFP cDNA, mouse acrosin promoter, mouse su9 cDNA, coral DsRed2 cDNA
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Commun Biol Apr 7;5(1):332.(2022) doi: 10.1038/s42003-022-03289-w.RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasahito Ikawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (TBUSAMTA_Nonprofit_BROAD)
  4. CAGGS MTA (English)
  5. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data