Strain Data Sheet

RBRC11237

Strain Information

Image
BRC No.RBRC11237
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameB6D2-Rsph6a<em3Osb>
Former Common nameRsph6a<em3/wt>
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2020. Fertilized eggs derived from BDF1 × BDF1 were used to generate this line. Mixed genetic background.
Strain descriptionRsph6a mutant (S17A, S20A) generated by the CRISPR/Cas9.
Colony maintenance
References
CRISPR/CAS9-mediated amino acid substitution reveals phosphorylation residues of RSPH6A are not essential for male fertility in micedagger.
Miyata H, Abbasi F, Visconti P E, Ikawa M
Biol. Reprod., 103(5):912-914 (2020). 32901808

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Rsph6a
MGI:1927643		radial spoke head 6 homolog A (Chlamydomonas)7Rsph6a<em3Osb> endonuclease-mediated mutation 3, Research Institute for Microbial Diseases, Osaka University

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNAStreptococcus pyogenes crRNA, tracrRNA, Mousea part of Rsph6a gene
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biol Reprod. 2020 Sep 9;ioaa161. doi: 10.1093/biolre/ioaa161. Online ahead of printRECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasahito Ikawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Co-creation Bureau, Osaka University HP

BRC mice in Publications

No Data