Strain Data Sheet
RBRC11223
Strain Information | |
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| Image | |
| BRC No. | RBRC11223 |
| Type | CRISPR/Cas9 |
| Species | Mus musculus |
| Strain name | STOCK Del(2Cstl1-Cstdc2)1Osb |
| Former Common name | (Cstl1-Cstdc2)<del/wt> |
| H-2 Haplotype | |
| ES Cell line | EGR-G01 [129S2 x C57BL/6NCr-Tg(CAG/Acr-EGFP)] |
| Background strain | |
| Appearance | |
| Strain development | Deposited by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2020. EGR-G01 ES cells derived from (129S2 x B6Cr)F1 were used. Mice were crossed to BDF1. Mixed genetic background. |
| Strain description | Deletion mutant of 114 kb on chromosome 2 covering Cstl1, Cst11, Cstdc1, Cst12, Cst8, Cst13, Cst9 and Cstdc2. |
| Colony maintenance | |
| References | Identification of multiple male reproductive tract-specific proteins that regulate sperm migration through the oviduct in mice. Fujihara Y, Noda T, Kobayashi K, Oji A, Kobayashi S, Matsumura T, Larasati T, Oura S, Kojima-Kita K, Yu Z, Matzuk M M, Ikawa M Proc. Natl. Acad. Sci. USA, 116(37):18498-18506 (2019). 31455729 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Cstdc2 MGI:1924955 | cystatin domain containing 2 | 2 | Cstdc2 | ||||
| Cstl1 MGI:2652834 | cystatin-like 1 | 2 | Cstl1 MGI:228756 | ||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | human hU6 promoter, CMV CMV enhancer (CBh), chicken chicken beta-actin promoter (CBh), SV40 SV40 nuclear localization signal, Streptococcus pyogenes SpCas9 (human codon-optimized), Escherichia coli ampicillin resistance gene, Streptomyces alboniger puromycin resistant gene, Mouse a part of Cstl1 gene,Mouse a part of Cstdc2 gene |
| Research application | |
| Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 116(37):18498-18506 (2019). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. |
| Depositor | Masahito Ikawa (Osaka University) |
| Strain Status |  Frozen sperm |
| Strain Availability |  Recovery and QC required prior to distribution |
| Additional Info. | Necessary documents for ordering:
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BRC mice in Publications |
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| No Data |