Strain Data Sheet

RBRC11221

Strain Information

Image
BRC No.RBRC11221
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameB6D2-Irgc1<em1Osb>
Former Common nameIrgc1<del/wt>
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2020. Fertilized eggs derived from BDF1 × BDF1 were used to generate this line. Mixed genetic background. Homozygous males show decreased fertility.
Strain descriptionIrgc1 KO mouse generated by the CRISPR/Cas9. 1266 bp deletion in the Irgc1 gene.
Colony maintenance
References
IRGC1, a testis-enriched immunity related GTPase, is important for fibrous sheath integrity and sperm motility in mice.
Yuki Kaneda, Haruhiko Miyata, Keisuke Shimada, Yuki Oyama, Rie Iida-Norita, Masahito Ikawa
Dev. Biol., S0012-1606(22)00099-9 (2022). 35618043

Health Report

Examination Date / Room / Rack2025/04/07Room:3-BRack:ASentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Irgc1
MGI:2685948		immunity-related GTPase family, cinema 17Irgc1<em1Osb> endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNAStreptococcus pyogenes crRNA, tracrRNA, Mouse a part of Irgc1 gene
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Dev. Biol., S0012-1606(22)00099-9 (2022). doi: 10.1016/j.ydbio.2022.05.011.RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasahito Ikawa (Osaka University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Co-creation Bureau, Osaka University HP
Genotyping protocol -PCR-

BRC mice in Publications

No Data