Strain Data Sheet

RBRC11219

Strain Information

Image
BRC No.RBRC11219
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameB6D2-C2cd6<em1Osb>
Former Common nameC2cd6<-43602/wt>
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2020. Fertilized eggs derived from BDF1 × BDF1 were used to generate this line. Mixed genetic background. Homozygous males are infertile.
Strain descriptionC2cd6 KO mouse generated by the CRISPR/Cas9. 43,602 bp deletion in the C2cd6 gene.
Colony maintenance
References
C2cd6-encoded CatSperτ targets sperm calcium channel to Ca2+ signaling domains in the flagellar membrane.
Jae Yeon Hwang, Huafeng Wang, Yonggang Lu, Masahito Ikawa, Jean-Ju Chung
Cell Rep., 38(3):110226 (2022). 34998468

C2cd6-encoded CatSperτ targets sperm calcium channel to Ca2+ signaling domains in the flagellar membrane.
Hwang J Y, Wang H, Lu Y, Ikawa M, Chung J J
Cell Rep., 38(3):110226 (2022). 34998468

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
C2cd6
MGI:1920713		C2 calcium dependent domain containing 61C2cd6<em1Osb> endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNAStreptococcus pyogenes crRNA, tracrRNA, Mouse a part of C2cd6 gene
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cell Rep. 2022 Jan 18;38(3):110226. doi: 10.1016/j.celrep.2021.110226. Epub 2022 Jan 7.RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasahito Ikawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Co-creation Bureau, Osaka University HP

BRC mice in Publications

No Data