Strain Data Sheet

RBRC11217

Strain Information

Image
BRC No.RBRC11217
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameSTOCK Kif9<em2Osb> Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb
Former Common nameKif9<LD/WT>
H-2 Haplotype
ES Cell lineEGR-G01 [129S2 x C57BL/6NCr-Tg(CAG/Acr-EGFP)]
Background strain
Appearance
Strain developmentDeposited by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2020. Mice were generated using EGR-G01 (ES cell) derived from (129S2 x B6Cr)F1-Tg(CAG/Acr-EGFP). Mice were further crossed to B6D2F1. Mixed genetic background.
Strain descriptionKif9 knockout mouse generated by the CRISPR/Cas9. 41,902 bp deletion in the Kif9 gene. This stain also has a transgene, Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb.
Colony maintenance
References
Testis-enriched kinesin KIF9 is important for progressive motility in mouse spermatozoa.
Miyata H, Shimada K, Morohoshi A, Oura S, Matsumura T, Xu Z, Oyama Y, Ikawa M
FASEB J., 34(4):5389-5400 (2020). 32072696

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Kif9
MGI:1098237		kinesin family member 99Kif9<em2Osb> endonuclease-mediated mutation 2, Research Institute for Microbial Diseases, Osaka University
EGFP Enhanced Green Fluorescent Protein (Aequorea victoria)UNEGFP CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), preproacrsin promoter

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNAhuman hU6 promoter, CMV CMV enhancer (CBh), chicken chicken beta-actin promoter (CBh), SV40 SV40 nuclear localization signal, Streptococcus pyogenes SpCas9 (human codon-optimized), crRNA, tracrRNA, Escherichia coli ampicillin resistance gene, Streptomyces alboniger puromycin resistant gene, Mousea part of Kif9 gene, Rabbit beta-globin polyadenylation signal, Mouse proacrosin signal peptide, Mouse acrosin N-ternimal peptide, Jellyfish GFP cDNA, Mouse acrosin promoter
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. FASEB J. 2020 Apr;34(4):5389-5400. doi: 10.1096/fj.201902755RRECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasahito Ikawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. CAGGS MTA (English)
  5. Acceptance of responsibility for living modified organism (Japanese / English)
Co-creation Bureau, Osaka University HP

BRC mice in Publications

No Data