Strain Data Sheet

RBRC11206

Strain Information

Image
BRC No.RBRC11206
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S2-Ramp1<tm1.2Tsuj>
Former Common nameRamp1 flox/+
H-2 Haplotype
ES Cell lineD3 [129S2/SvPas]
Background strain
Appearance
Strain developmentDeveloped and deposited by Kazutake Tsujikawa, Osaka University in 2020. ES cells derived from 129/Sv. Mice were backcrossed to C57BL/6 for 12 generations. C57BL/6 congenic line.
Strain descriptionRAMP1 (receptor activity-modifying protein 1) floxed mice. A pair of loxP flanks exon 2.
Colony maintenance
ReferencesProc. Natl. Acad. Sci. USA, 104, 16702-16707 (2007). 17923674

Health Report

Examination Date / Room / Rack2024/01/22Room:3-CRack:H
2023/10/23Room:3-CRack:H
2023/07/24Room:3-CRack:H
2023/04/24Room:3-CRack:H
2023/01/23Room:3-CRack:H
2022/10/25Room:3-CRack:H
2022/07/25Room:3-CRack:H

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Ramp1receptor (calcitonin) activity modifying protein 11Ramp1<tm1.2Tsuj>targeted mutation 1.2, Kazutake Tsujikawa9130218E19Rik

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP1loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP1loxP

Ordering Information

Donor DNAphage P1 loxP sites, Mouse Ramp1 genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc Natl Acad Sci USA. 2007 Oct 16;104(42):16702-7. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorKazutake Tsujikawa (Osaka University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data