Strain Data Sheet

RBRC11202

Strain Information

Image
BRC No.RBRC11202
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameSTOCK Slc12a2<tm1.1(tetO)Kftnk>
Former Common nameNKCC1 tetO knockin
H-2 Haplotype
ES Cell lineG0G1
Background strain
Appearance
Strain development寄託者が作出。G0G1 (阪大微研の岡部勝先生が樹立, 129とB6のhybrid) で作成。129SvEvTac、C57BL6Jなどとのmixed background。tetO homozygoteは野生型として振る舞う。Actin-tTSと交配すると、難聴になる。
Strain descriptionNKCC1遺伝子の翻訳開始前にtetOカセットがノックインしてある（Tanaka KF et al., Biol Psychiatry 2010の方法に従う）。野生型マウスとして使用できる。tTAマウスと組み合わせればNKCC1過剰発現を、tTSマウスと組み合わせればNKCC1ノックダウンが可能になる（Watabe et al., Sci Rep 2017）
Colony maintenance
ReferencesNat. Commun., 12(1) 5146 (2021). 34446732
Sci. Rep., 7(1):13605. (2017). 29051615

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Slc12a2solute carrier family 12, member 218Slc12a2<tm1.1(tetO)Kftnk>targeted mutation 1.1, Kenji Tanaka

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site18Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP18loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP18loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
tetOtetracycline operator (E. coli)18tetO

Ordering Information

供与核酸phage P1 loxP sites, yeast FRT (flippase recombination target) site, tetracycline operator (E. coli), Cytomegalovirus CMV minimum promoter, mouse Slc12a2 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Tet system
提供条件条件を付加しない。
Depositor田中　謙二（慶應義塾大学）
Strain Status凍結精子のアイコン凍結精子
Strain Availability凍結精子より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data