Strain Data Sheet

RBRC11200

Strain Information

Image
BRC No.RBRC11200
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Bdnf<tm1(STOP-tetO)Kftnk>
Former Common nameBDNF STOP-tetO knockin
H-2 Haplotype
ES Cell lineG0G1
Background strain
Appearance
Strain development寄託者が作出。G0G1 (阪大微研の岡部勝先生が樹立, 129とB6のhybrid) で作成。C57BL6Jに5代以上backcrossしてある。
Strain descriptionSTOP-tetOが、BDNF geneのexon 4にノックインされている（Tanaka KF et al., Biol Psychiatry 2010の方法に従う）。Isoform 4, 5,6,7の発現が抑制されている（Sano et al., Sci Rep 2019）。
Colony maintenance
ReferencesSci. Rep., 9(1):3422. (2019). 30833600

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Bdnfbrain derived neurotrophic factor2Bdnf<tm1(tetO)Kftnk>targeted mutation 1, Kenji Tanaka

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site2Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP2loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)2mouse Phosphoglycerate kinase promoter (PGK promoter), synthetic bacterial EM7 promoter (T7 phage)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
tetOtetracycline operator (E. coli)2tetO

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA2

Ordering Information

供与核酸マウス PGK プロモーター, synthetic bacterial EM7 promoter (T7 phage), 大腸菌 Neo 耐性遺伝子, 酵母His3 3’UTR, SV40 ウイルス SV40 polyA シグナル, 酵母 FRT配列, 大腸菌Tetracycline operator site, サイトメガロウイルス CMV最小プロモーター, P1ファージ loxP配列
Research applicationCre/loxP system
FLP/frt system
Tet system
提供条件条件を付加しない。
Depositor田中　謙二（慶應義塾大学）
Strain Status凍結胚のアイコン凍結胚
Strain Availability受注後、検査を施して提供
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data