Strain Data Sheet

RBRC11196

Strain Information

Image
BRC No.RBRC11196
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6-Kit<tm1.1(EGFP)Shkm>
Former Common namec-Kit<EGFP> knock-in mice
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Shigeo Koyasu in 2021. This strain was generated using B6JN/1 ES cells. The neo gene cassette was removed by crossing to B6;SJL-Tg(ACTFLPE)9205Dym/J. Homozygous mice are infertile.
Strain descriptionKnock-in mice in which EGFP was placed at the c-Kit gene locus.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Kitkit oncogene5Kit<tm1.1(EGFP)Shkm>targeted mutation 1.1, Shigeo Koyasu

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)5EGFP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site5Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA5

Ordering Information

Donor DNAオワンクラゲ EGFP（GFPの変異体）, Saccharomyces cerevisiae Flp recombination Target 配列 (FRT), Simian virus 40 (SV40) polyA付加配列
Research applicationFLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorShigeo Koyasu (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data