Strain Data Sheet

RBRC11196

Strain Information

Image
BRC No.RBRC11196
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6-Kit<tm1.1(EGFP)Shkm>
Former Common namec-Kit<EGFP> knock-in mice
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Shigeo Koyasu in 2021. This strain was generated using B6JN/1 ES cells. The neo gene cassette was removed by crossing to B6;SJL-Tg(ACTFLPE)9205Dym/J. Homozygous mice are infertile.
Strain descriptionKnock-in mice in which EGFP was placed at the c-Kit gene locus.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
EGFP Enhanced Green Fluorescent Protein (Aequorea victoria)5EGFP
Frt yeast FRT (flippase recombination target) site5Frt
Kit
MGI:96677		KIT proto-oncogene receptor tyrosine kinase5Kit<tm1.1(EGFP)Shkm> targeted mutation 1.1, Shigeo Koyasu
  • acute myeloid leukemia(DisGeNET, MedGEN)

  • cutaneous mastocytosis(MedGEN)

  • gastrointestinal stromal tumor(DisGeNET, MedGEN)
    • more 3 Diseases
  • mastocytosis(DisGeNET)

  • piebaldism(DisGeNET, MedGEN)

  • testicular germ cell tumor(MedGEN)
    • SV40 polyA5

    Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
    Detailed phenotype data

    Ordering Information

    Donor DNAオワンクラゲ EGFP（GFPの変異体）, Saccharomyces cerevisiae Flp recombination Target 配列 (FRT), Simian virus 40 (SV40) polyA付加配列
    Research applicationFLP/frt system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorShigeo Koyasu (RIKEN)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovery and QC required prior to distribution
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    BRC mice in Publications

    No Data