Strain Data Sheet

RBRC11195

Strain Information

Image
BRC No.RBRC11195
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6-Il1rl1<tm1.1(hCD2/YFP)Shkm>
Former Common nameT1/ST2<hCD2-YFP> knock-in mice
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Shigeo Koyasu in 2021. This strain was generated using B6JN/1 ES cells. The neo gene cassette was removed by crossing to B6;SJL-Tg(ACTFLPE)9205Dym/J.
Strain descriptionKnock-in mice in which a fusion gene of human CD2 and YFP was placed at the IL1RL1 gene locus.
Colony maintenance
References

Health Report

Examination Date / Room / Rack2024/01/09Room:3-ARack:C
2023/12/21Room:3-ARack:C
2023/10/10Room:3-ARack:C
2023/07/10Room:3-ARack:C
2023/04/10Room:3-ARack:C
2023/01/10Room:3-ARack:C
2022/10/11Room:3-ARack:C
2022/07/11Room:3-ARack:C
2022/06/06Room:3-ARack:C

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Il1rl1interleukin 1 receptor-like 11Il1rl1<tm1.1(hCD2/YFP)Shkm>targeted mutation 1.1, Shigeo Koyasu

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
CD2CD2 (human)1CD2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EYFPEnhanced Yellow Fluorescent Protein (Aequorea victoria)1EYFP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site1Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA1

Ordering Information

Donor DNAhuman CD2 cDNA, jellyfish EYFP cDNA (GFP variant), Simian virus 40 (SV40) polyA additional signal, Saccharomyces cerevisiae Flp recombination Target site (FRT) site, mouse Il1rl1 genomic DNA
Research applicationFLP/frt system
Fluorescent Proteins/lacZ System
Immunology and Inflammation Research
Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorShigeo Koyasu (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data