Strain Data Sheet

RBRC11185

Strain Information

Image
BRC No.RBRC11185
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Gt(ROSA)26Sor<tm1.1(CAG-tdiRFP713NLS)Imayo>
Former Common nameR26-CAG-LoxP-iRFP713NLS
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development2018年、今吉　格(Itaru Imayoshi)／京都大学大学院生命科学研究科（Graduate School of Biostudies, Kyoto University）が奥野 浩行／鹿児島大学大学院医歯学総合研究科（Graduate School of Medical and Dental Sciences, Kagoshima University）との共同研究で作成。C57BL/6N由来のES細胞(EGR-108)での相同組換えにより作製。
Strain description蛍光たんぱく質tdiFP713NLSが、Cre及びFLPの両方の組み換え後に発現するコンストラクトを、Rosa26遺伝子座にノックインしてある。発現レベルを増強する為に、プロモーターはCAGプロモーターを用いており、またtdiFP713NLSの終止コドンとSV40pA配列の間にWPRE配列を挿入してある。キメラマウスをpCAG-FLPeマウスと交配し、FRT-Stop-FRTカセットを除去した。tdiFP713NLSにはHA-tagが付加してある。
Colony maintenance
References

Health Report

Examination Date / Room / Rack2024/03/11Room:3-2Rack:D
2023/12/11Room:3-2Rack:D
2023/09/11Room:3-2Rack:D
2023/06/13Room:3-2Rack:D
2023/03/13Room:3-2Rack:D
2022/12/12Room:3-2Rack:D

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1.1(CAG-tdiRFP713NLS)Imayo>targeted mutation 1.1, Itaru Imayoshi

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site6Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GHGrowth hormone polyA (Bovine)6GH

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HAhuman influenza virus HA (hemagglutinin) tag sequence6HA

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
T2AThosea asigna virus 2A peptide (insect)6T2A

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
WPREWoodchuck hepatitis virus posy-transcriptional regulatory element (WPRE)6WPRE

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
iRFP713iRFP713 (humanized iRFP713)6iRFP713

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
iRFP713iRFP713 (humanized iRFP713)6iRFP713

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)6mouse Phosphoglycerate kinase promoter (PGK promoter), phage T7 gb2 promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
nlsSV40 nuclear localaization signal (chemically-synthesized nucleotide)6nls

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
nlsSV40 nuclear localaization signal (chemically-synthesized nucleotide)6nls

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
tdiRFP713tdiRFP713 (tandem dimeric iRFP713)6tdiRFP713CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA6

Ordering Information

供与核酸CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, chicken beta-actin intron, rabbit beta-globin intron), Bacteriophage P1 loxP sites, mouse Phosphoglycerate kinase promoter (PGK promoter), phage T7 gb2 promoter, E. coli neomycin resistance gene, pA, bovine GH polyA, SV40 polyA signal, rabbit beta-globin polyA, Yeast FRT (flippase recombination target) site, Near-InfraRed Fluorescent Protein (Rhodopseudomonas palustris) tdiRFP713 (tandem dimeric iRFP713), SV40 nuclear localaization signal (chemically-synthesized nucleotide), Thosea asigna virus 2A peptide (insect), human influenza virus HA (hemagglutinin) tag sequence, Woodchuck hepatitis virus posy-transcriptional regulatory element (WPRE)
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
提供条件条件を付加する。
研究成果の公表にあたって寄託者の指定する文献を引用する。(will be announced.)
学術機関の学術研究に限る。分与希望者は事前に今吉格(E-mail:imayoshi.itaru.2n@kyoto-u.ac.jp)に連絡をとり、分与に関して許可を得ること。 (提供承諾書(書式6)の提出は不要)。
Depositor今吉　格（国立大学法人京都大学）
Strain Status生体のアイコン生体
凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結精子を1ヶ月以内に提供可能
凍結胚を1ヶ月以内に提供可能
生体マウスを1~3ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. CAGGS MTA (English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data