Strain Data Sheet

RBRC11130

Strain Information

Image
BRC No.RBRC11130
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Tubb3<tm1.1(Cre)Ksak>
Former Common nameTubb3-Cre
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strain
Appearance
Strain development新潟大学・崎村建司（2005）RENKA C57BL/6N-derived ES cells (Mishina and Sakimura,2007)を用いて作出。C57BL/6N(チャールスリバー)
Strain descriptiontubulin, beta 3 class III（MGI:107813）の遺伝子座に組換え酵素Cre、IRES配列、蛍光タンパク質EGFPをノックインしたマウス。神経細胞にCreを発現（EGFPの発現は検出感度以下）。FLP/frt組換えによりNeoカセットは除去済み。神経科学研究に有用。
Colony maintenance
ReferencesCell Rep., 36(4):109427(2021). 34320360

Health Report

Examination Date / Room / Rack2024/03/11Room:3-5Rack:B
2023/12/11Room:3-5Rack:B
2023/09/13Room:3-5Rack:B
2023/09/11Room:3-5Rack:B
2023/06/13Room:3-5Rack:B
2023/03/13Room:3-5Rack:B
2022/12/12Room:3-5Rack:B
2022/09/12Room:3-5Rack:B
2022/06/13Room:3-5Rack:B

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Tubb3tubulin, beta 3 class III8Tubb3

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EGFPGreen Fluorescent Protein (Aequorea victoria)8EGFP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site8Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Iresinternal ribosomal entry site (EMCV)8

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
crePhage P1 Cre recombinase8cre

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
nlsSV40 large T antigen nuclear localization signal (NLS)8nls

Ordering Information

供与核酸SV40 large T antigen nuclear localization signal (NLS), Bacteriophage P1 Cre recombinase, EMCV internal ribosomal entry site (IRES), Aequorea victoria EGFP cDNA, Saccharomyces cerevisiae Frt site, mouse Tubb3 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
提供条件条件を付加する。
研究成果の公表にあたって寄託者の指定する文献を引用する。Cell Rep., 36(4):109427(2021).
学術機関の学術研究に限る。
Depositor崎村　建司（国立大学法人新潟大学）
Strain Status生体のアイコン生体
凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結精子を1ヶ月以内に提供可能
生体マウスを3~6ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data