Strain Data Sheet

RBRC11119

Strain Information

Image
BRC No.RBRC11119
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6-Tlx1<tm2.1Rygo>
Former Common nameTlx1<fl>
H-2 Haplotype
ES Cell lineEGR-101 [C57BL/6N]
Background strain
Appearance
Strain developmentDeposited by Ryo Goitsuka, Tokyo, Tokyo University of Science, in 2020. Mice were generated using ES cell line, EGR-101. C57BL/6J genetic background.
Strain descriptionTlx1 floxed mice. Exon 1 is flanked by a pair of loxP. The neo resistance gene was removed from this strain.
Colony maintenance
ReferencesSci. Rep., 8, 1-16 (2018). 29844356

Health Report

Examination Date / Room / Rack2023/11/27Room:4-BRack:E
2023/08/30Room:4-BRack:E
2023/08/28Room:4-BRack:E
2023/05/29Room:4-BRack:E
2023/03/22Room:4-BRack:E
2023/02/27Room:4-BRack:E
2022/11/29Room:4-BRack:E
2022/08/29Room:4-BRack:E

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Tlx1T cell leukemia, homeobox 119Tlx1<tm2.1Rygo>targeted mutation 2.1, Ryo Goitsuka

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site19Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP19loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP19loxP

Ordering Information

Donor DNAphage P1 loxP sites, yeast FRT (flippase recombination target) site, mouse Tlx1 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Sci. Rep., 8, 1-16 (2018).
DepositorRyo Goitsuka (Tokyo University of Science)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data