Strain Data Sheet

RBRC11118

Strain Information

Image
BRC No.RBRC11118
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6-Tlx1<tm1.1(cre/ERT2/ires2/Venus)Rygo>
Former Common nameTlx1<CreER-Venus>
H-2 Haplotype
ES Cell lineEGR-101 [C57BL/6N]
Background strain
Appearance
Strain developmentEGR-101 C57BL/6 strain-derived ES cells provided by Dr. M.Okabe of Osaka University.Heterozygote x Homozygote [or Crossing to C57BL/6JJmsSlc
Strain descriptionTlx1遺伝子の翻訳開始コドンを含む第一エキソンにCreERT2-ires2-Venus遺伝子カセットをノックインしたマウス。Homozygoteは脾臓を欠損する。
Colony maintenance
ReferencesSci. Rep., 8, 1-16 (2018). 29844356
Genesis, 52(11) 916-23 (2014). 25283275
Sci. Rep., 9, 20408 (2019). 31892733

Health Report

Examination Date / Room / Rack2024/02/19Room:4-ARack:L
2023/11/20Room:4-ARack:L

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Tlx1T cell leukemia, homeobox 119Tlx1<tm1.1(cre/ERT2/ires2/Venus)Rygo>targeted mutation 1.1, Ryo Goitsuka

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
ERT2estrogen receptor (ER), human ER with the triple mutation G400V/M543A/L544A)19ERT2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site19Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Iresinternal ribosomal entry site (EMCV)19

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
VenusYellow Fluorescent Protein (Aequorea victoria)19Venus

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
crePhage P1 Cre recombinase19cre

Ordering Information

供与核酸Phage P1 Cre recombinase, human estrogen receptor (ER) cDNA, EMCV IRES2, jellyfish Yellow Fluorescent Protein (Aequorea victoria) cDNA, yeast FRT (flippase recombination target) site
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
提供条件条件を付加する。
研究成果の公表にあたって寄託者の指定する文献を引用する。Genesis, 52(11) 916-23 (2014).
研究成果の公表にあたってVenusの開発者である宮脇敦史先生への謝辞の表明並びに指定する文献を引用する。Nat. Biotechnol., 20, 87-90 (2002).
Depositor後飯塚　僚（東京理科大学）
Strain Status生体のアイコン生体
凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結精子を1ヶ月以内に提供可能
生体マウスを3~6ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data