Strain Data Sheet
RBRC11117
Strain Information | |
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| Image | |
| BRC No. | RBRC11117 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6.B6129-Gt(ROSA)26Sor<tm1(CAG-HA/Tlx1)Rygo> |
| Former Common name | Rosa26<Tlx1> |
| H-2 Haplotype | |
| ES Cell line | IDG26.10-3 |
| Background strain | |
| Appearance | |
| Strain development | Deposited by Ryo Goitsuka, Tokyo, Tokyo University of Science, in 2020. Mice were generated using ES cell line IDG26.10-3 (Hitz, C., Wurst, W. & Kuhn, R. Conditional brain-specific knockdown of MAPK using Cre/loxP regulated RNA interference. Nucleic Acids Res 35, e90,2007). C57BL/6J congenic strain by backcrossing for 12 generations. |
| Strain description | Conditional Tlx1 expressing mice by Cre recombinase. CAG-loxP-stop-loxP HA-tagged Tlx1 is integrated at the ROSA26 locus. |
| Colony maintenance | |
| References | Niche-induced extramedullary hematopoiesis in the spleen is regulated by the transcription factor Tlx1. Oda A, Tezuka T, Ueno Y, Hosoda S, Amemiya Y, Notsu C, Kasahara T, Nishiyama C, Goitsuka R Sci. Rep., 8, 1-16 (2018). 29844356 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| GH | Growth hormone polyA (Bovine) | 6 | GH | ||||
| Gt(ROSA)26Sor MGI:104735 | gene trap ROSA 26, Philippe Soriano | 6 | Gt(ROSA)26Sor<tm1(CAG-HA/Tlx1)Rygo> | targeted mutation 1, Ryo Goitsuka | |||
| HA | human influenza virus HA (hemagglutinin) tag sequence | 6 | HA | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) | |||
| Tlx1 | T cell leukemia, homeobox 1 | 6 | Tlx1 | ||||
| attL | attachment site | 6 | attL | ||||
| attR | attachment site | 6 | attR | ||||
| loxP | phage P1 loxP | 6 | loxP | ||||
| neo | neomycin resistance gene (E. coli) | 6 | mouse phosphoglycerate kinase promoter (PGK promoter) | ||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | ヒトインフルエンザウイルス HA-tag, 大腸菌 Neomycin 耐性遺伝子, マウス PGKプロモーター, サイトメガロウイルス、ニワトリ、ウサギ CAG プロモーター（cytomegalovirus early enhancer, chicken beta actin promoter and rabbit beta-globin gene）, ウシ bovine growth hormone polyadenylation site (bpA), P1ファージ loxP 配列, gamma ファージ attR配列, gamma ファージ attL配列 |
| Research application | Cre/loxP system |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Sci. Rep., 8, 1-16 (2018). |
| Depositor | Ryo Goitsuka (Tokyo University of Science) |
| Strain Status |  Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering: |
BRC mice in Publications |
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| No Data |