Strain Information | |
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Image | |
BRC No. | RBRC11100 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.129-Elovl1<tm1.1Akih> |
Former Common name | Elovl1 KO |
H-2 Haplotype | |
ES Cell line | E14 [129P2/OlaHsd] |
Background strain | |
Appearance | |
Strain development | Deposited by Akio Kihara, Hokkaido University, in 2020. Mice were generated using E14 (ES cell) derived from 129P2/OlaHsd. Mice were further backcrossed to C57BL/6J for more than 21 generations. |
Strain description | Elovl1 knockout mice. A loxP site was inserted upstream of exon 2. An FRT-flanked neomycin resistance cassette with a 5' loxP site was inserted downstream of exon 8. Cre-mediated recombination removed exons 2 through 8 and left the FRT-flanked neomycin resistance cassette intact. |
Colony maintenance | |
References | Mol. Cell. Biol., 33, 2787-2796 (2013). 23689133 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Elovl1elongation of very long chain fatty acids (FEN1/Elo2, SUR4/Elo3, yeast)-like 14Elovl1<tm1.1Akih>targeted mutation 1.1, Akio Kihara Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site4Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site4Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP4loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)4mouse Phosphoglycerate kinase promoter (PGK promoter), synthetic bacterial EM7 promoter (T7 phage) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyA4 |
Ordering Information | |
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Donor DNA | Bacteriophage P1 loxP site, Saccharomyces cerevisiae FRT sites, synthetic bacterial EM7 promoter (T7 phage), Mouse Phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistant gene, SV40 PolyA signal, mouse Elovl1 genomic DNA |
Research application | Cre/loxP system FLP/frt system |
Specific Term and Conditions | In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
Depositor | Akio Kihara (Hokkaido University) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |