Strain Data Sheet

RBRC11094

Information1

Image
BRC No.RBRC11094
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameC57BL/6J-Ddx4<em1(cre/ERT2)Utr>
Former Common nameB6-Ddx4<em1(CreERT2)Utr>
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Seiya Mizuno, University of Tuskuba in 2020. This strain was generated with the CRISPR/Cas9. C57BL/6J background.
Strain descriptionCre/ERT2 knock-in mice at the C-terminal portion of the Ddx4 gene via 2A peptide. Homozygous males are infertile.
Colony maintenance
ReferencesGenesis, 58(7):e23367 (2020). 32293787

Health Report

Examination Date / Room / Rack2023/02/20Room:4-ARack:K
2022/11/21Room:4-ARack:K

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Ddx4DEAD (Asp-Glu-Ala-Asp) box polypeptide 413Ddx4<em1(cre/ERT2)Utr>endonuclease-induced mutation 1, University of Tsukuba Laboratory Animal Resource Center

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
ERT2estrogen receptor (ER), human ER with the triple mutation G400V/M543A/L544A)13ERT2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HBB2rabbit(Oryctolagus cuniculus) beta globin polyA13HBB2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
P2Aporcine teschovirus-1 2A peptide13P2A

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
crePhage P1 Cre recombinase13cre

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
nlsSV40 large T antigen nuclear localization signal (NLS)13nls

Information2

Donor DNAP2A (porcine teschovirus-1 2A peptide), Simian virus 40 nuclear localization signal (NLS), bacteriophage P1 Cre recombinase, human (Homo sapiens)estrogen receptor 1 (alpha) cDNA, rabbit(Oryctolagus cuniculus) beta globin polyA
Research applicationCre/loxP system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genesis, 58(7):e23367 (2020).
DepositorSeiya Mizuno (University of Tsukuba)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (3 to 6 months)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data