Strain Data Sheet

RBRC11094

Strain Information

Image
BRC No.RBRC11094
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameC57BL/6J-Ddx4<em1(cre/ERT2)Utr>
Former Common nameB6-Ddx4<em1(CreERT2)Utr>
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Seiya Mizuno, University of Tuskuba in 2020. This strain was generated with the CRISPR/Cas9. C57BL/6J background.
Strain descriptionCre/ERT2 knock-in mice at the C-terminal portion of the Ddx4 gene via 2A peptide. Homozygous males are infertile.
Colony maintenance
References
Generation of B6-Ddx4em1(CreERT2)Utr , a novel CreERT2 knock-in line, for germ cell lineage by CRISPR/Cas9.
Hoai Thu Le, Yoshikazu Hasegawa, Yoko Daitoku, Kanako Kato, Saori Miznuo-Iijima, Tra Thi Huong Dinh, Yumeno Kuba, Yuki Osawa, Natsuki Mikami, Kento Morimoto, Shinya Ayabe, Yoko Tanimoto, Kazuya Murata, Ken-Ichi Yagami, Satoru Takahashi, Seiya Mizuno, Fumihiro Sugiyama
Genesis, 58(7):e23367 (2020). 32293787

Health Report

Examination Date / Room / Rack2025/12/22Room:4-ARack:KSentinel mouse program
2025/10/14Room:4-ARack:KSentinel mouse program
2025/07/28Room:4-ARack:KSentinel mouse program
2025/05/19Room:4-ARack:KSentinel mouse program
2025/02/17Room:4-ARack:KSentinel mouse program
2024/11/18Room:4-ARack:KSentinel mouse program
2024/08/19Room:4-ARack:KSentinel mouse program
2024/05/20Room:4-ARack:KSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Ddx4
MGI:102670		DEAD box helicase 413Ddx4<em1(cre/ERT2)Utr> endonuclease-induced mutation 1, University of Tsukuba Laboratory Animal Resource Center
ERT2 estrogen receptor (ER), human ER with the triple mutation G400V/M543A/L544A)13ERT2
HBB2 rabbit(Oryctolagus cuniculus) beta globin polyA13HBB2
P2A porcine teschovirus-1 2A peptide13P2A
cre Phage P1 Cre recombinase13cre
nls SV40 large T antigen nuclear localization signal (NLS)13nls

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • infertility (MP:0001924)
    • Detailed phenotype data

    Ordering Information

    Donor DNAP2A (porcine teschovirus-1 2A peptide), Simian virus 40 nuclear localization signal (NLS), bacteriophage P1 Cre recombinase, human (Homo sapiens)estrogen receptor 1 (alpha) cDNA, rabbit(Oryctolagus cuniculus) beta globin polyA
    Research applicationCre/loxP system
    Cre driver
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genesis, 58(7):e23367 (2020).
    DepositorSeiya Mizuno (University of Tsukuba)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Live mouse (3 to 6 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
    3. Cre-ERT(2): NOTICE TO RECIPIENT
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Oct 2022

    BRC mice in Publications

    No Data