Strain Data Sheet

RBRC11064

Strain Information

Image
BRC No.RBRC11064
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameB6D2-Dnah8<em1Osb>
Former Common nameDnah8 KO, Dnah8<del/wt>
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2020. Fertilized eggs derived from BDF1 × BDF1 were used to generate this line. Mixed genetic background.
Strain descriptionDnah8 knockout mouse generated by the CRISPR/Cas9. 239,922 bp deletion and 4 bp insertion in the Dnah8 gene.
Colony maintenance
References
Bi-allelic DNAH8 Variants Lead to Multiple Morphological Abnormalities of the Sperm Flagella and Primary Male Infertility.
Liu C, Miyata H, Gao Y, Sha Y, Tang S, Xu Z, Whitfield M, Patrat C, Wu H, Dulioust E, Tian S, Shimada K, Cong J, Noda T, Li H, Morohoshi A, Cazin C, Kherraf Z E, Arnoult C, Jin L, He X, Ray P F, Cao Y, Toure A, Zhang F, Ikawa M
Am. J. Hum. Genet., 107(2):330-341 (2020). 32619401

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Dnah8
MGI:107714		dynein, axonemal, heavy chain 817Dnah8<em1Osb> endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University
  • spermatogenic failure 46(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
    Detailed phenotype data

    Ordering Information

    Donor DNAStreptococcus pyogenes crRNA, tracrRNA, Mouse a part of Dnah8 gene
    Research application
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Am. J. Hum. Genet., 107(2):330-341 (2020). doi: 10.1016/j.ajhg.2020.06.004.The RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
    DepositorMasahito Ikawa (Osaka University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovery and QC required prior to distribution
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP, Co-creation Bureau, Osaka University HP

    BRC mice in Publications

    No Data