Strain Data Sheet

RBRC11059

Strain Information

Image
BRC No.RBRC11059
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameB6D2-Glt6d1<em2Osb> 4930568D16Rik<em1Osb> 4930402F06Rik<em1Osb>
Former Common nameGlt6dl paralog TKO, Glt6d1/4930568D16Rik/4930402F06Rik TKO
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2020. Fertilized eggs derived from BDF1 × BDF1 were used to generate this line. Mixed genetic background.
Strain descriptionTriple mutant mouse deleting 8 bp in the Glt6d, 10 bp in the 4930568D16Rik and 163 bp in the 4930402F06Rik.
Colony maintenance
ReferencesBiol. Reprod., 103(2):195-204 (2020). doi: 10.1093/biolre/ioaa084. 32561905

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Glt6d1glycosyltransferase 6 domain containing 12Glt6d1<em2Osb>endonuclease-mediated mutation 2, Research Institute for Microbial Diseases, Osaka University

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
4930402F06RikRIKEN cDNA 4930402F06 gene24930402F06Rik<em1Osb>endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
4930568D16RikRIKEN cDNA 4930568D16 gene24930568D16Rik<em1Osb>endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University

Ordering Information

Donor DNAStreptococcus pyogenes crRNA, tracrRNA, human hU6 promoter, CMV enhancer (CBh), chicken beta-actin promoter (CBh), SV40 nuclear localization signal, Streptococcus pyogenes SpCas9 (human codon-optimized), crRNA, tracrRNA, Escherichia coli ampicillin resistant gene, Mousea part of Glt6d1 gene, Mouse a part of 4930568D16Rik gene, Mouse a part of 4930402F06Rik gene
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biol. Reprod., 103(2):195-204 (2020). doi: 10.1093/biolre/ioaa084.The RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasahito Ikawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP

BRC mice in Publications

No Data