Strain Data Sheet

RBRC11057

Strain Information

Image
BRC No.RBRC11057
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameC57BL/6J-Phf7<em4Osb>
Former Common namePhf7 KO, Phf7<-11929/wt>
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2020. Fertilized eggs derived from C57BL/6J were used to generate this line. C57BL/6J genetic background.
Strain descriptionPhf7 knockout mouse generated by the CRISPR/Cas9. 11,929 bp deletion in the Phf7 gene.
Colony maintenance
References
PHF7 Modulates BRDT Stability and Histone-to-Protamine Exchange during Spermiogenesis.
Kim C R, Noda T, Kim H, Kim G, Park S, Na Y, Oura S, Shimada K, Bang I, Ahn J Y, Kim Y R, Oh S K, Choi H J, Kim J S, Jung I, Lee H, Okada Y, Ikawa M, Baek S H
Cell Rep., 32(4):107950 (2020). 32726616

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Phf7
MGI:1919088		PHD finger protein 714Phf7<em4Osb> endonuclease-mediated mutation 4, Research Institute for Microbial Diseases, Osaka University

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNAStreptococcus pyogenes crRNA, tracrRNA, Mouse a part of Phf7 gene
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cell Rep., 32(4):107950 (2020). doi: 10.1016/j.celrep.2020.107950.The RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasahito Ikawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP, Co-creation Bureau, Osaka University HP

BRC mice in Publications

No Data