Strain Information | |
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Image | |
BRC No. | RBRC11056 |
Type | CRISPR/Cas9 (Transgene) |
Species | Mus musculus |
Strain name | C57BL/6J-Phf7<em3(FLAG/Phf7)Osb> |
Former Common name | FLAG-Phf7 KI, Phf7<Flag/wt> |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Deposited by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2020. Fertilized eggs derived from C57BL/6J were used to generate this line. C57BL/6J genetic background. |
Strain description | Flag tag knockin mice at N-terminus of Phf7 gene generated by the CRISPR/Cas9. |
Colony maintenance | |
References | Cell Rep., 32(4):107950 (2020). doi: 10.1016/j.celrep.2020.107950. 32726616 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Phf7PHD finger protein 714Phf7<em3(FLAG/Phf7)Osb>endonuclease-mediated mutation 3, Research Institute for Microbial Diseases, Osaka University Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter FLAGFLAG tag (synthetic)14FLAG |
Ordering Information | |
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Donor DNA | Streptococcus pyogenes crRNA, tracrRNA, Mouse a part of Phf7 gene, synthetic FLAG tag, Escherichia coli Lac Z, T7 phage T7 promoter, Escherichia coli lac promoter, Escherichia coli ampicillin resistant gene |
Research application | |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cell Rep., 32(4):107950 (2020). doi: 10.1016/j.celrep.2020.107950.The RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. |
Depositor | Masahito Ikawa (Osaka University) |
Strain Status | Frozen sperm |
Strain Availability | Recovery and QC required prior to distribution |
Additional Info. | Necessary documents for ordering:
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BRC mice in Publications |
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No Data |