Strain Information | |
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Image | |
BRC No. | RBRC11022 |
Type | CRISPR/Cas9 |
Species | Mus musculus |
Strain name | B6D2-Cklf<em1Osb> |
Former Common name | Cklf <-6257+1/wt> |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Deposited by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2020. Fertilized eggs derived from BDF1 × BDF1 were used to generate this line. Mixed genetic background. |
Strain description | Cklf KO mouse generated by the CRISPR/Cas9. 6257 bp deletion and 1 bp addition in the Cklf gene. Homozygous mutants are embryonically lethal. |
Colony maintenance | |
References | Proc. Natl. Acad. Sci. USA, 118 (35) e2106673118 (2021). 34446558 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Cklfchemokine-like factor8Cklf<em1Osb>endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University |
Ordering Information | |
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Donor DNA | Streptococcus pyogene scrRNA, tracrRNA, Mousea part of Cklf gene |
Research application | |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PNAS. August 31,118 (35) e2106673118;(2021) https://doi.org/10.1073/pnas.2106673118The RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. |
Depositor | Masahito Ikawa (Osaka University) |
Strain Status | Frozen sperm |
Strain Availability | Recovery and QC required prior to distribution |
Additional Info. | Necessary documents for ordering:
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BRC mice in Publications |
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No Data |