Strain Data Sheet

RBRC11022

Strain Information

Image
BRC No.RBRC11022
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameB6D2-Cklf<em1Osb>
Former Common nameCklf <-6257+1/wt>
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2020. Fertilized eggs derived from BDF1 × BDF1 were used to generate this line. Mixed genetic background.
Strain descriptionCklf KO mouse generated by the CRISPR/Cas9. 6257 bp deletion and 1 bp addition in the Cklf gene. Homozygous mutants are embryonically lethal.
Colony maintenance
References
SPATA33 localizes calcineurin to the mitochondria and regulates sperm motility in mice.
Miyata H, Oura S, Morohoshi A, Shimada K, Mashiko D, Oyama Y, Kaneda Y, Matsumura T, Abbasi F, Ikawa M
Proc. Natl. Acad. Sci. USA, 118 (35) e2106673118 (2021). 34446558

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Cklf
MGI:1922708		chemokine-like factor8Cklf<em1Osb> endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNAStreptococcus pyogene scrRNA, tracrRNA, Mousea part of Cklf gene
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PNAS. August 31,118 (35) e2106673118;(2021) https://doi.org/10.1073/pnas.2106673118The RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasahito Ikawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP, Co-creation Bureau, Osaka University HP

BRC mice in Publications

No Data