Strain Data Sheet

RBRC11008

Strain Information

Image
BRC No.RBRC11008
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameB6D2-Del(2Lcn8-Lcn10)1Osb Lcn9<em2Osb>
Former Common nameLcn8-10<del/wt> Lcn9<del/wt>
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2020. Fertilized eggs derived from BDF1 × BDF1 were used to generate this line. Mixed genetic background.
Strain descriptionDeletion mutant in 2 loci on chromosome 2 generated by the CRISPR/Cas9. 32521 bp deletion from Lcn8 to Lcn10 and 1894 bp deletion in the Lcn9 gene.
Colony maintenance
ReferencesAndrology. doi: 10.1111/andr.13350. (2022). 36428102

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Lcn9lipocalin 92Lcn9<em2Osb>endonuclease-mediated mutation 2, Research Institute for Microbial Diseases, Osaka University

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Lcn10lipocalin 102Lcn10

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Lcn8lipocalin 82Lcn8

Ordering Information

Donor DNAStreptococcus pyogenes crRNA, tracrRNA, Mouse a part of Lcn8 gene, Mouse a part of Lcn10 gene, Mousea part of Lcn9 gene
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Andrology. doi: 10.1111/andr.13350. (2022).The RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasahito Ikawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP

BRC mice in Publications

No Data