Strain Information | |
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Image | |
BRC No. | RBRC10999 |
Type | CRISPR/Cas9 |
Species | Mus musculus |
Strain name | STOCK-Prr27<em1Osb> |
Former Common name | Prr27<-21011+1/wt> |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Deposited by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2020. EGR-G01 ES cells derived from (129S2 x B6Cr)F1 were used. Mice were crossed to BDF1. Mixed genetic background. Male homozygous mutants tend to exhibit infertility. |
Strain description | Prr27 KO mouse generated by the CRISPR/Cas9. 21011 bp deletion and 1bp addition in the Prr27 gene. |
Colony maintenance | |
References |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Prr27proline rich 275Prr27<em1Osb>endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University |
Ordering Information | |
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Donor DNA | human hU6 promoter, CMV enhancer (CBh), chicken chicken beta-actin promoter (CBh), SV40 nuclear localization signal, Streptococcus pyogenes SpCas9 (human codon-optimized), crRNA, tracrRNA, Escherichia coli ampicillin resistant gene, Streptomyces alboniger puromycin resistant gene, Mouse a part of Prr27 gene |
Research application | |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. |
Depositor | Masahito Ikawa (Osaka University) |
Strain Status | Frozen sperm |
Strain Availability | Recovery and QC required prior to distribution |
Additional Info. | Necessary documents for ordering:
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BRC mice in Publications |
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No Data |