Strain Data Sheet

RBRC10992

Strain Information

Image
BRC No.RBRC10992
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(Prnp-TARDBP*R208)#Koya
Former Common namePrP-TDP43 R208
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped and deposited by Dr. Koji Yamanaka. Tg mice were generated using BDF1 and backcrossed to C57BL/6J for more than 10 generations.
Strain descriptionTransgenic mice expressing mutated human TARDBP (R208) protein with 3x FLAG tag at C-terminal under the control of mouse Prnp promoter.
Colony maintenance
ReferencesSci. Rep., 7(1):14972 (2017). 29097807

Health Report

Examination Date / Room / Rack2023/03/13Room:3-5Rack:D
2022/12/12Room:3-5Rack:D
2022/09/12Room:3-5Rack:D
2022/06/13Room:3-5Rack:D

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
TARDBPTAR DNA binding protein (human)UNTARDBP

Ordering Information

Donor DNAmouse prion genomic DNA, human TDP-43 gene and mutant(TDP-43-R208), FLAG tag
Research applicationNeurobiology Research
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Sci. Rep., 7(1):14972 (2017).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. Otherwise, the availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT must understand the intellectual property of the MoPrP vector is owned by Johns Hopkins University. Therefore, an acknowledgment for use of the MoPrP vector to Johns Hopkins University is requested.
DepositorKoji Yamanaka (Nagoya University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data