Strain Information | |
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Image | |
BRC No. | RBRC10969 |
Type | CRISPR/Cas9 (Transgene) |
Species | Mus musculus |
Strain name | B6(Cg)-T<em1(dre)Okn> |
Former Common name | Triple lineagelabelling mice (brachyury2A-Dre Delta Puro) |
H-2 Haplotype | |
ES Cell line | B6NJ-22<UTR> [C57BL/6NCrlCrlj] |
Background strain | |
Appearance | |
Strain development | Deposited by Hideyuki Okano, Keio University in 2020. C57BL/6 genetic background. |
Strain description | Knock-in mice of Dre at the C-terminus of protein coding sequence of the T gene. |
Colony maintenance | |
References | Development, 146(21). pii: dev174938 (2019). 31597657 |
Health Report | |
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Examination Date / Room / Rack | 2024/02/26Room:4-BRack:H 2023/11/27Room:4-BRack:H 2023/08/30Room:4-BRack:H 2023/08/28Room:4-BRack:H 2023/05/29Room:4-BRack:H 2023/02/27Room:4-BRack:H 2023/02/20Room:4-BRack:H 2022/11/29Room:4-BRack:H 2022/08/29Room:4-BRack:H 2022/05/31Room:4-BRack:H |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter DreBacteriophage D6 recombinase17Dre Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter F3yeast FRT (flippase recombination target) site (synthetic) variant17F3 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter P2Aporcine teschovirus-1 2A peptide17P2A Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter nlsSV40 large T antigen nuclear localization signal (NLS)17nls |
Ordering Information | |
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Donor DNA | mouse T/brachyury genomic DNA, P2A (porcine teschovirus-1 2A peptide), P1-like transducing phage D6 Dre, SV40 virus NLS, Saccharomyces cerevisia FRT variant(F3) |
Research application | Cre/loxP system FLP/frt system |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development. 2019 Nov 4;146(21). pii: dev174938 |
Depositor | Hideyuki Okano (Keio University) |
Strain Status | Live mice Frozen sperm |
Strain Availability | Cryopreserved sperm (within 1 month) Live mouse (1 to 3 months) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |