Strain Data Sheet

RBRC10958

Strain Information

Image
BRC No.RBRC10958
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameC57BL/6-Gcg<em1(cre)Utr>
Former Common nameGcg-Cre
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentGcg遺伝子の3’末に2A配列とともにCre遺伝子を挿入している。C57BL/6J(SLC)マウスと交配。
Strain descriptionGcg遺伝子発現細胞でのCre組換え酵素の発現、Gcg陽性細胞での遺伝子組換え。この系統だけでは表現型はない。
Colony maintenance
ReferencesExp. Anim., 69(2):178-188 (2020). doi: 10.1538/expanim.19-0105. 31787710

Health Report

Examination Date / Room / Rack2024/03/11Room:3-3Rack:A
2023/12/11Room:3-3Rack:A
2023/09/11Room:3-3Rack:A
2023/06/13Room:3-3Rack:A
2023/03/13Room:3-3Rack:A
2022/12/12Room:3-3Rack:A
2022/09/12Room:3-3Rack:A
2022/06/13Room:3-3Rack:A

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gcgglucagon2Gcg<em1(cre)Utr>endonuclease-induced mutation 1, University of Tsukuba Laboratory Animal Resource Center

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HBB2rabbit bata-globin polyA2HBB2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
P2Aporcine teschovirus-1 2A peptide2P2A

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
crePhage P1 Cre recombinase2cre

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
nlsSV40 large T antigen nuclear localization signal (NLS)2nls

Ordering Information

供与核酸P2A (porcine teschovirus-1 2A peptide), SV40 nls, P1 phage Cre recombinase, rabbit beta-globin polyA
Research applicationCre/loxP system
提供条件条件を付加する。
研究成果の公表にあたって寄託者の指定する文献を引用する。Exp Anim. 2019 Dec 2. doi: 10.1538/expanim.19-0105.
Depositor高橋　智（国立大学法人筑波大学）
Strain Status生体のアイコン生体
凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結精子を1ヶ月以内に提供可能
生体マウスを1~3ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data