Strain Information | |
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Image | |
BRC No. | RBRC10929 |
Type | CRISPR/Cas9 (Transgene) |
Species | Mus musculus |
Strain name | C57BL/6N-Emilin2<em1Suna> |
Former Common name | Emilin2 KO |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed and deposited by Sunao Takeshita, National Center for Geriatrics and Gerontolog in 2019. This strain was generated with the CRISPR/Cas9. C57BL/6N genetic background. |
Strain description | Emilin2 mutant mice with one base deletion at exon 1 of the Emilin2 gene. |
Colony maintenance | |
References |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Emilin2elastin microfibril interfacer 217Emilin2<em1Suna>endonuclease-mediated mutation 1, Sunao Takeshita |
Ordering Information | |
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Donor DNA | mouse Emilin2, Streptococcus pyogenes Cas9*, jellyfish GFP cDNA*, Escherichia coli Kanamycin resistance gene, CMV Promoter, immediate early enhancer, U6 gRNA T7 2A pUC origin* Not detected by PCR using Marker Gene Detection kit (TOYOBO, Osaka, Japan). Other introduced genes were not tested. |
Research application | Fluorescent Proteins/lacZ System |
Specific Term and Conditions | In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
Depositor | Sunao Takeshita (National Center for Geriatrics and Gerontology) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |