Strain Data Sheet

RBRC10929

Strain Information

Image
BRC No.RBRC10929
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameC57BL/6N-Emilin2<em1Suna>
Former Common nameEmilin2 KO
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped and deposited by Sunao Takeshita, National Center for Geriatrics and Gerontolog in 2019. This strain was generated with the CRISPR/Cas9. C57BL/6N genetic background.
Strain descriptionEmilin2 mutant mice with one base deletion at exon 1 of the Emilin2 gene.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Emilin2elastin microfibril interfacer 217Emilin2<em1Suna>endonuclease-mediated mutation 1, Sunao Takeshita

Ordering Information

Donor DNAmouse Emilin2, Streptococcus pyogenes Cas9*, jellyfish GFP cDNA*, Escherichia coli Kanamycin resistance gene, CMV Promoter, immediate early enhancer, U6 gRNA T7 2A pUC origin* Not detected by PCR using Marker Gene Detection kit (TOYOBO, Osaka, Japan). Other introduced genes were not tested.
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorSunao Takeshita (National Center for Geriatrics and Gerontology)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data