Strain Data Sheet
RBRC10927
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC10927 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | C57BL/6N-Aldoc<tm1(tdTomato)Ksak> |
| Former Common name | Aldoc-tdTomato KI |
| H-2 Haplotype | |
| ES Cell line | RENKA [C57BL/6NCrlCrlj] |
| Background strain | |
| Appearance | |
| Strain development | Developed by Kenji Sakimura, Niigata University in 2015. RENKA ES cells derived from C57BL/6N were used to generate the mutant mice. |
| Strain description | tdTomato reporter strain knocked in at the translation initiation codon of the Aldoc gene. C57BL/6N genetic background. |
| Colony maintenance | Heterozygote x Wild-type [or Crossing to C57BL/6NCrl] |
| References | Structure-function relationships between aldolase C/zebrin II expression and complex spike synchrony in the cerebellum. Tsutsumi S, Yamazaki M, Miyazaki T, Watanabe M, Sakimura K, Kano M, Kitamura K J. Neurosci., 35(2):843-52 (2015). 25589776 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Aldoc | aldolase C, fructose-bisphosphate | 11 | Aldoc | targeted mutation 1, Kenji Sakimura | |||
| loxP | phage P1 loxP | 11 | loxP | ||||
| loxP | phage P1 loxP | 11 | loxP | ||||
| neo | neomycin resistance gene (E. coli) | 11 | mouse Phosphoglycerate kinase promoter (PGK promoter), phage T7 gb2 promoter | ||||
| tdTomato | tandem dimeric Red Fluorescent Protein (Discosoma sp.) | 11 | tdTomato | ||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | Discosoma sp. tdTomato gene, Bacteriophage P1 loxP sites, Mouse Phosphoglycerate kinase (PGK) promoter, phage T7 gb2 promoter, E. coli Neomycin resistant gene, mouse Aldoc genomic DNA |
| Research application | Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurosci., 35(2):843-52 (2015). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. |
| Depositor | Kenji Sakimura (Niigata University) |
| Strain Status |  Live mice Frozen sperm |
| Strain Availability |  Recovery and QC required prior to distributionCryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |