Strain Data Sheet

RBRC10927

Strain Information

Image
BRC No.RBRC10927
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Aldoc<tm1(tdTomato)Ksak>
Former Common nameAldoc-tdTomato KI
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strain
Appearance
Strain developmentDeveloped by Kenji Sakimura, Niigata University in 2015. RENKA ES cells derived from C57BL/6N were used to generate the mutant mice.
Strain descriptiontdTomato reporter strain knocked in at the translation initiation codon of the Aldoc gene. C57BL/6N genetic background.
Colony maintenanceHeterozygote x Wild-type [or Crossing to C57BL/6NCrl]
References
Structure-function relationships between aldolase C/zebrin II expression and complex spike synchrony in the cerebellum.
Tsutsumi S, Yamazaki M, Miyazaki T, Watanabe M, Sakimura K, Kano M, Kitamura K
J. Neurosci., 35(2):843-52 (2015). 25589776

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Aldoc
MGI:101863		aldolase C, fructose-bisphosphate11Aldoc<tm1(tdTomato)Ksak>
MGI:6324252		targeted mutation 1, Kenji Sakimura
loxP phage P1 loxP11loxP
loxP phage P1 loxP11loxP
neo neomycin resistance gene (E. coli)11 mouse Phosphoglycerate kinase promoter (PGK promoter), phage T7 gb2 promoter
tdTomato tandem dimeric Red Fluorescent Protein (Discosoma sp.)11tdTomato

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
Detailed phenotype data

Ordering Information

Donor DNADiscosoma sp. tdTomato gene, Bacteriophage P1 loxP sites, Mouse Phosphoglycerate kinase (PGK) promoter, phage T7 gb2 promoter, E. coli Neomycin resistant gene, mouse Aldoc genomic DNA
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurosci., 35(2):843-52 (2015). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
DepositorKenji Sakimura (Niigata University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: TBUSAMTA Nonprofit
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data