Strain Information | |
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Image | |
BRC No. | RBRC10866 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.Cg-Hras<tm1.1(G12S)Tumg> |
Former Common name | HrasG12S/+ (Hras G12S knock-in mice; C57BL/6J background) |
H-2 Haplotype | |
ES Cell line | Bruce 4 [B6.Cg-Thy1<a>] |
Background strain | |
Appearance | |
Strain development | Developed by Yoko Aoki and Shin-ichi Inoue, Tohoku University School of Medicine in 2018. BRUCE-4 ES cells were used. C57BL/6 congenic line. |
Strain description | Knock-in mutants in which a point mutation (G12S) was introduced at Exon 1 of the Hras gene. Floxed cDNA/stop cassette and Frt-flnaked Neo cassette were removed by crossing with Cre mice. BALB/C congenic strain is also available as RBRC11578. |
Colony maintenance | |
References | Mice with an Oncogenic HRAS Mutation are Resistant to High-Fat Diet-Induced Obesity and Exhibit Impaired Hepatic Energy Homeostasis. Oba D, Inoue S I, Miyagawa-Tomita S, Nakashima Y, Niihori T, Yamaguchi S, Matsubara Y, Aoki Y EBioMedicine, 27:138-150 (2018). 29254681 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
Hras | Harvey rat sarcoma virus oncogene | 7 | Hras | targeted mutation 1.1, Yoko Aoki | |||
loxP | phage P1 loxP | 7 | loxP |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | Bacteriophage P1 loxP site, mouse Hras genomic DNA |
Research application | Apoptosis Research Cancer Research Cardiovascular Research Cre/loxP system Hematological Research Metabolism Research Mouse Models for Human Disease |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. EBioMedicine, 27:138-150 (2018). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICALRESOURCE. |
Depositor | Yoko Aoki (Tohoku University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Mouse of the Month Nov 2022 |
BRC mice in Publications |
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No Data |