Strain Data Sheet

RBRC10866

Strain Information

Image
BRC No.RBRC10866
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Hras<tm1.1(G12S)Tumg>
Former Common nameHrasG12S/+ (Hras G12S knock-in mice; C57BL/6J background)
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strain
Appearance
Strain developmentDeveloped by Yoko Aoki and Shin-ichi Inoue, Tohoku University School of Medicine in 2018. BRUCE-4 ES cells were used. C57BL/6 congenic line.
Strain descriptionKnock-in mutants in which a point mutation (G12S) was introduced at Exon 1 of the Hras gene. Floxed cDNA/stop cassette and Frt-flnaked Neo cassette were removed by crossing with Cre mice. BALB/C congenic strain is also available as RBRC11578.
Colony maintenance
References
Mice with an Oncogenic HRAS Mutation are Resistant to High-Fat Diet-Induced Obesity and Exhibit Impaired Hepatic Energy Homeostasis.
Oba D, Inoue S I, Miyagawa-Tomita S, Nakashima Y, Niihori T, Yamaguchi S, Matsubara Y, Aoki Y
EBioMedicine, 27:138-150 (2018). 29254681

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
HrasHarvey rat sarcoma virus oncogene7Hrastargeted mutation 1.1, Yoko Aoki
  • Costello syndrome(DisGeNET, MedGEN)

  • large congenital melanocytic nevus(MedGEN)

  • linear nevus sebaceous syndrome(MedGEN)
  • more 4 Diseases
  • melanoma(DisGeNET)

  • nevus, epidermal(MedGEN)

  • thyroid cancer, nonmedullary, 2(MedGEN)

  • urinary bladder cancer(MedGEN)
  • loxPphage P1 loxP7loxP
  • Costello syndrome(DisGeNET, MedGEN)

  • large congenital melanocytic nevus(MedGEN)

  • linear nevus sebaceous syndrome(MedGEN)
  • more 4 Diseases
  • melanoma(DisGeNET)

  • nevus, epidermal(MedGEN)

  • thyroid cancer, nonmedullary, 2(MedGEN)

  • urinary bladder cancer(MedGEN)
  • Phenotype

    Annotation by Mammalian phenotyhpe ontology
    Detailed phenotype data

    Ordering Information

    Donor DNABacteriophage P1 loxP site, mouse Hras genomic DNA
    Research applicationApoptosis Research
    Cancer Research
    Cardiovascular Research
    Cre/loxP system
    Hematological Research
    Metabolism Research
    Mouse Models for Human Disease
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. EBioMedicine, 27:138-150 (2018). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICALRESOURCE.
    DepositorYoko Aoki (Tohoku University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Nov 2022

    BRC mice in Publications

    No Data