Strain Data Sheet

RBRC10850

Strain Information

Image
BRC No.RBRC10850
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;B6129-Tet3<tm1.1Hko>
Former Common nameTET3
H-2 Haplotype
ES Cell lineM1 [(C57BL/6 x 129)F1]
Background strain
Appearance
Strain development理化学研究所　古関明彦(2011年)M1(B6,129)のES細胞を用いて作成。数回B6と交配し、その後兄妹交配。
Strain descriptionTet3 gene floxed mice. Gene targeting for the Tet3 allele was done by flanking exons 8 and 9 with Cre-loxP sequences. Conditional deletion of Tet3 (exons 8 and 9) can be achieved by crossing the mice with specific cre deleters such as CreERT2. The TET3 protein is a dioxygenase that catalyzes the conversion of 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and further oxidized cytosine (5fC and 5caC), and plays a key role in active DNA demethylation. Gdf9-Cre-driven Tet3 deletion in early oocytes resulted in a complete absence of Tet3 messenger RNA in Tet3mat-MII oocytes and Tet3 protein in zygotes (Amouroux et al., 2016). This results in a lack of 5hmC formation in the paternal pronucleus following fertilization in zygotes. This conditional allele would be useful to study the roles of Tet3 in DNA de-methylation by combining with tissue specific Cre deleters.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Tet3tet methylcytosine dioxygenase 36Tet3<tm1.1Hko>targeted mutation 1.1, Haruhiko Koseki

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site6Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Ordering Information

供与核酸P1 phage loxP sites, yeast FRT (flipase recombination target) site, mouse Tet3 genomic DNA
Research applicationCre/loxP system
FLP/frt system
提供条件条件を付加する。利用者は提供承諾書を用いて、事前に寄託者の承諾を得る。
Depositor古関　明彦（国立研究開発法人理化学研究所）
Strain Status凍結精子のアイコン凍結精子
Strain Availability凍結精子より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data