Strain Data Sheet

RBRC10849

Strain Information

Image
BRC No.RBRC10849
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;B6129-Tet2<tm1.1Hko>
Former Common nameTET2
H-2 Haplotype
ES Cell lineM1 [(C57BL/6 x 129)F1]
Background strain
Appearance
Strain development理化学研究所　古関明彦(2011年)M1(B6,129)のES細胞を用いて作成。数回B6と交配し、その後兄妹交配。
Strain descriptionTet2 gene floxed mice. Gene targeting for the Tet2 allele was done by flanking exon 9 with Cre-loxP sequences. Conditional deletion of Tet2 (exon 9) can be achieved by crossing the mice with specific cre deleters such as CreERT2. The TET2 protein is a dioxygenase that catalyzes the conversion of 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and further oxidized cytosine (5fC and 5caC), and plays a key role in active DNA demethylation. This conditional allele would be useful to study the roles of Tet2 in DNA de-methylation by combining with tissue specific Cre deleters.
Colony maintenance
References

Health Report

Examination Date / Room / Rack2024/01/09Room:3-BRack:D
2023/10/10Room:3-BRack:D
2023/07/10Room:3-BRack:D
2023/04/10Room:3-BRack:D
2023/01/10Room:3-BRack:D
2022/10/11Room:3-BRack:D
2022/07/11Room:3-BRack:D

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Tet2tet methylcytosine dioxygenase 23Tet2<tm1.1Hko>targeted mutation 1.1, Haruhiko Koseki

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site3Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP3loxP

Ordering Information

供与核酸yeast FRT (flippase recombination target) site, phage P1 loxP site, mouse Tet2 genomic DNA
Research applicationCre/loxP system
FLP/frt system
提供条件条件を付加する。利用者は提供承諾書を用いて、事前に寄託者の承諾を得る。
Depositor古関　明彦（国立研究開発法人理化学研究所）
Strain Status生体のアイコン生体
凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結精子を1ヶ月以内に提供可能
生体マウスを3~6ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data