Strain Data Sheet

RBRC10849

Strain Information

Image
BRC No.RBRC10849
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;B6129-Tet2<tm1.1Hko>
Former Common nameTET2
H-2 Haplotype
ES Cell lineM1 [(C57BL/6 x 129)F1]
Background strain
Appearance
Strain developmentDeveloped by Haruhiko Koseki, RIKEN in 2011. M1 ((129 x C57BL/6)F1) ES cells were used. This strain was backcrossed to C57BL/6J.
Strain descriptionTet2 gene floxed mice. Gene targeting for the Tet2 allele was done by flanking exon 9 with Cre-loxP sequences. Conditional deletion of Tet2 (exon 9) can be achieved by crossing the mice with specific cre deleters such as CreERT2. The TET2 protein is a dioxygenase that catalyzes the conversion of 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and further oxidized cytosine (5fC and 5caC), and plays a key role in active DNA demethylation. This conditional allele would be useful to study the roles of Tet2 in DNA de-methylation by combining with tissue specific Cre deleters.
Colony maintenance
References

Health Report

Examination Date / Room / Rack2024/01/09Room:3-BRack:D
2023/10/10Room:3-BRack:D
2023/07/10Room:3-BRack:D
2023/04/10Room:3-BRack:D
2023/01/10Room:3-BRack:D
2022/10/11Room:3-BRack:D
2022/07/11Room:3-BRack:D

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Tet2tet methylcytosine dioxygenase 23Tet2<tm1.1Hko>targeted mutation 1.1, Haruhiko Koseki

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site3Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP3loxP

Ordering Information

Donor DNAyeast FRT (flippase recombination target) site, phage P1 loxP site, mouse Tet2 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
DepositorHaruhiko Koseki (RIKEN)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Live mouse (3 to 6 months)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data