Strain Data Sheet
RBRC10821
Strain Information | |
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| Image | |
| BRC No. | RBRC10821 |
| Type | CRISPR/Cas9 (Transgene) |
| Species | Mus musculus |
| Strain name | B6D2-Pabpn1l<em1Osb> |
| Former Common name | Pabpn1l-13/-13 |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | Deposited by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2019. Fertilized eggs derived from BDF1 × BDF1 were used to generate this line. Mixed genetic background. |
| Strain description | Pabpn1l<em1Osb>; 13 bp deletion at the Pabpn1l locus. |
| Colony maintenance | |
| References | CRISPR/Cas9-mediated genome editing reveals 30 testis-enriched genes dispensable for male fertility in micedagger. Lu Y, Oura S, Matsumura T, Oji A, Sakurai N, Fujihara Y, Shimada K, Miyata H, Tobita T, Noda T, Castaneda J M, Kiyozumi D, Zhang Q, Larasati T, Young S A M, Kodani M, Huddleston C A, Robertson M J, Coarfa C, Isotani A, Aitken R J, Okabe M, Matzuk M M, Garcia T X, Ikawa M Biol. Reprod., 101(2):501-511 (2019). 31201419PABPN1L is required for maternal mRNA degradation after meiosis resumption. Chihiro Emori, Mayo Kodani, Ferheen Abbasi, Masashi Mori, Masahito Ikawa J. Reprod. Dev., (2023). 38057116 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Pabpn1l MGI:2685954 | poly(A)binding protein nuclear 1-like | 8 | Pabpn1l<em1Osb> | endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University | |||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | pX330-U6-Chimeric_BB-CBh-hSpCas9[human U6 promoter, S. pyogenes gRNA scaffold, human U6 terminator, CMV,chicken hybrid CMV enhancer/chicken beta-actin promoter (CBh), Synthetic DNA 3xFLAG, SV40 nuclear localization signal(NLS), Streptococcus pyogenes SpCas9 (human codon-optimized), bovine GH polyA signal, AAV2 inverted terminal repeat (ITR), f1 phage f1 origin, E. coli Ampicillin resistance gene (AmpR), E. coli pUC origin], Mouse a part of Pabpn1l gene |
| Research application | |
| Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biol. Reprod., 101(2):501-511 (2019).The RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. |
| Depositor | Masahito Ikawa (Osaka University) |
| Strain Status |  Frozen sperm |
| Strain Availability |  Recovery and QC required prior to distribution |
| Additional Info. | Necessary documents for ordering:
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BRC mice in Publications |
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| No Data |