Strain Data Sheet

RBRC10819

Strain Information

Image
BRC No.RBRC10819
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameSTOCK Bpifa3<em1Osb>
Former Common nameBpifa3-8110/-8110
H-2 Haplotype
ES Cell lineEGR-G01 [129S2 x C57BL/6NCr-Tg(CAG/Acr-EGFP)]
Background strain
Appearance
Strain developmentDeposited by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2019. EGR-G01 ES cells derived from (129S2 x B6Cr)F1 were used. Mice were crossed to BDF1. Mixed genetic background.
Strain descriptionBpifa3<em1Osb>; 8110 bp deletion at the Bpifa3 locus.
Colony maintenance
References
CRISPR/Cas9-mediated genome editing reveals 30 testis-enriched genes dispensable for male fertility in micedagger.
Lu Y, Oura S, Matsumura T, Oji A, Sakurai N, Fujihara Y, Shimada K, Miyata H, Tobita T, Noda T, Castaneda J M, Kiyozumi D, Zhang Q, Larasati T, Young S A M, Kodani M, Huddleston C A, Robertson M J, Coarfa C, Isotani A, Aitken R J, Okabe M, Matzuk M M, Garcia T X, Ikawa M
Biol. Reprod., 101(2):501-511 (2019). 31201419

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Bpifa3
MGI:1920638		BPI fold containing family A, member 32Bpifa3<em1Osb> endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNAhuman hU6 promoter CMV enhancer (CBh) chicken chicken beta-actin promoter (CBh) SV40 nuclear localization signal Streptococcus pyogenes SpCas9 (human codon-optimized), crRNA, tracrRNA Escherichia coli ampicillin resistance gene Streptomyces alboniger puromycin resistant gene Mouse a part of Bpifa3 gene
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biol. Reprod., 101(2):501-511 (2019).The RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasahito Ikawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP, Co-creation Bureau, Osaka University HP

BRC mice in Publications

No Data