Strain Data Sheet

RBRC10762

Strain Information

Image
BRC No.RBRC10762
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.B6CB-Gsdmd<tm1.2Tshir>/Ms
Former Common nameGsdmdflox/flox, Gsdmdfl/fl, Gsdmd cKO
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Toshihiko Shiroishi and Tomoaki Fujii, National Institute of Genetics in 2005. TT2 ES cells derived from (C57BL/6 x CBA)F1 were used. Mice were backcrossed to C57BL/6J for more than 10 generations. C57BL/6 congenic line. Frt-flanked Neo cassette was deleted by crossing with Flp mice.
Strain descriptionFloxed mice for Gsdmd.
Colony maintenance
References
Gasdermin D (Gsdmd) is dispensable for mouse intestinal epithelium development.
Tomoaki Fujii, Masaru Tamura, Shigekazu Tanaka, Yoriko Kato, Hiromi Yamamoto, Youichi Mizushina, Toshihiko Shiroishi
Genesis, 46, 418-423 (2008). 18693275

Gasdermin D represses inflammation-induced colon cancer development by regulating apoptosis.
Shigekazu Tanaka, Hajime Orita, Taro Kataoka, Masahiro Miyazaki, Harumi Saeki, Ryo Wada, Malcolm V Brock, Tetsu Fukunaga, Takanori Amano, Toshihiko Shiroishi
Carcinogenesis, 44(4):341-349 (2023). 36753047

Health Report

Examination Date / Room / Rack2026/01/05Room:4-CRack:ESentinel mouse program
2025/10/20Room:4-CRack:ESentinel mouse program
2025/08/04Room:4-CRack:ESentinel mouse program
2025/05/26Room:4-CRack:ESentinel mouse program
2025/02/25Room:4-CRack:ESentinel mouse program
2024/11/25Room:4-CRack:ESentinel mouse program
2024/08/26Room:4-CRack:ESentinel mouse program
2024/05/27Room:4-CRack:ESentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site15Frt
Gsdmd
MGI:1916396		gasdermin D15Gsdmd<tm1.2Tshir> targeted mutation 1.2, Toshihiko Shiroishi
loxP phage P1 loxP15loxP
loxP phage P1 loxP15loxP

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • no abnormal phenotype detected (MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAP1 bacteriophage loxP sites, S. cerevisiae Frt site, mouse Gsdmd genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Immunology and Inflammation Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genesis, 46, 418-423 (2008). The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. (Contact info: Tsuyoshi Koide, Ph.D. <tkoide@nig.ac.jp>, Mouse Genomics Resource Laboratory)
    DepositorToshihiko Shiroishi (National Institute of Genetics)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Mar 2020

    BRC mice in Publications

    Yoon SH, Kim CY, Lee E, Lee C, Lee KS, Lee J, Park H, Choi B, Hwang I, Kim J, Kim TG, Son J, Hyun YM, Hong S, Yu JW.
    Microglial NLRP3-gasdermin D activation impairs blood-brain barrier integrity through interleukin-1β-independent neutrophil chemotaxis upon peripheral inflammation in mice.
    Nat Commun 16(1) 699(2025) 39814731
    Ma H, Jia H, Zou W, Ji F, Wang W, Zhao J, Yuan C, Jiao J.
    Gasdermin D Mediated Mitochondrial Metabolism Orchestrate Neurogenesis Through LDHA During Embryonic Development.
    Adv Sci (Weinh) 11(35) e2402285(2024) 39033542
    Shim DW, Eo JC, Kim S, Hwang I, Nam B, Shin JE, Han SH, Yu JW.
    Deficiency of circadian clock gene Bmal1 exacerbates noncanonical inflammasome-mediated pyroptosis and lethality via Rev-erbα-C/EBPβ-SAA1 axis.
    Exp Mol Med 56(2) 370-382(2024) 38297162