Strain Data Sheet

RBRC10757

Strain Information

Image
BRC No.RBRC10757
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Mab21l2<tm1Nao>
Former Common nameC57BL/6(Mab21l2tm1Nao), Mab21l2tm1Nao
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain development奈良先端科学技術大学院大学・高橋直樹 (2004年)R1 ES 細胞を用いて作出。C57BL/6コンジェニック系統。Mab21l2tm1Nao heterozygouteをC57BL/6J Jclに10世代戻し交配を行う。ホモ型胎生致死。
Strain descriptionThis gene is similar to the MAB-21 cell fate-determining gene found in C. elegans. It is required for several aspects of embryonic development including normal development of the eye, heart and body wall. Mutation details: The entire gene coding region was replaced with a neomycin resistance cassette via homologous recombination. Absence of gene expression in homozygous mutants was confirmed by whole mount in situ hybridization analysis of E10.5 homozygous mutant embryos.
Colony maintenance
ReferencesDev. Biol., 274(2):295-307 (2004). 15385160

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Mab21l2mab-21-like 23Mab21l2<tm1Nao>targeted mutation 1, Naoki Takahashi

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)3herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA3

Ordering Information

供与核酸herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli Neomycin resistant gene, SV40 PolyA signal, mouse Mab21l2 genomic DNA
Research application
提供条件条件を付加する。利用者は提供承諾書を用いて、事前に寄託者の承諾を得る。
Depositor高橋　直樹（国立大学法人東京大学）
Strain Status生体のアイコン生体
凍結精子のアイコン凍結精子
Strain Availability受注後、検査を施して提供
凍結精子を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data