Strain Data Sheet

RBRC10750

Strain Information

Image
BRC No.RBRC10750
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Lrrc4<tm3(KOr)Ito>
Former Common nameNgl2-kusabiraorange
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development理化学研究所　脳神経科学研究センター・糸原　重美らにより開発された。C57BL/6J由来。ES細胞：CMTI。C57BL/6J(日本クレア)で維持。IVFに使用したオスはN4。
Strain descriptionNgl2のSalにDTA(Diphtheria toxin A)を挿入し、細胞内領域の代わりにFRT-neo-FRT-mKusabiraOrangeを挿入したトランスジェニックマウス。
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Lrrc4leucine rich repeat containing 46Lrrc4<tm3(KOr)Ito>targeted mutation 3, Shigeyoshi Itohara

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site6Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
KOOrange Fluorescent Protein, Kusabira Orange (Verrillofungia concinna)6KO

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)6mouse Phosphoglycerate kinase promoter (PGK promoter), phage T7 gb2 promoter

Ordering Information

供与核酸マウス Ngl2, pgk, 出芽酵母 FRT配列, E.coli Neo耐性遺伝子, phage T7 gb2 promoter, サンゴ類 Kusabira-orange
Research applicationFLP/frt system
Fluorescent Proteins/lacZ System
提供条件条件を付加しない。
Depositor糸原　重美（国立研究開発法人理化学研究所）
Strain Status凍結胚のアイコン凍結胚
Strain Availability受注後、検査を施して提供
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data