Strain Data Sheet

RBRC10745

Strain Information

Image
BRC No.RBRC10745
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(tetO-YC2.60)#Ito
Former Common nameTRE-YC2.60
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development理化学研究所　脳神経科学研究センター・糸原重美らにより開発された。使用したオスはF0。
Strain descriptionTetオペレーターの制御下でYCレポーター（YC2.60）を発現するトランスジェニックマウス。
Colony maintenance
References

Health Report

Examination Date / Room / Rack2024/01/09Room:3-BRack:B
2023/10/10Room:3-BRack:B
2023/07/10Room:3-BRack:B

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
WPREWoodchuck hepatitis virus posy-transcriptional regulatory element (WPRE)UNWPRE

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
YC2.60Yellow Cameleon 2.60 (Aequorea victoria ECFP cDNA, Xenopus laevis calm2 cDNA, calmodulin (CaM)-binding peptide (rabbit skeletal muscle myosin light chain kinase (MLCK) cDNA), circularly permuted form of the Venus yellow fluorescent protein cDNA)UNYC2.60E. coli tetO (tetracycline operator)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 Intron polyAUN

Ordering Information

供与核酸E. coli tetO (tetracycline operator), Yellow Cameleon 2.60 (Aequorea victoria ECFP cDNA, Xenopus laevis calm2 cDNA, calmodulin (CaM)-binding peptide (rabbit skeletal muscle myosin light chain kinase (MLCK) cDNA), circularly permuted form of the Venus yellow fluorescent protein cDNA), Woodchuck hepatitis virus posttranscriptional regulatory element (WPRE), SV40 Intron polyA
Research applicationFluorescent Proteins/lacZ System
Tet system
提供条件条件を付加する。
研究成果の公表にあたってVenusの開発者である宮脇敦史先生への謝辞の表明並びに指定する文献を引用する。Nat. Biotechnol., 20, 87-90 (2002).
Depositor糸原　重美（国立研究開発法人理化学研究所）
Strain Status生体のアイコン生体
凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結精子を1ヶ月以内に提供可能
生体マウスを1~3ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER of the YC2.60 using Approval form (Form V (English)) (Lab Contact: Laboratory for Cell Function Dynamics, RIKEN CBS: mta-cfds@ml.riken.jp).
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-
Sequence of Y2.60

BRC mice in Publications

No Data