Strain Data Sheet

RBRC10744

Strain Information

Image
BRC No.RBRC10744
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Eno2<tm1(DTA)Hsak>/Ito
Former Common nameNSE-DTA
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Shigeyoshi Itohara at RIKEN.
Strain descriptionCre-dependent DTA expression cassettes was knocked in at the Eno2 locus.
Colony maintenance
ReferencesNature, 450(7169) 503-508 (2007). 17989651
Front. Behav. Neurosci., 7:17 (2013). 23487260

Health Report

Examination Date / Room / Rack2024/01/09Room:3-ARack:G
2023/10/16Room:3-ARack:G
2023/10/10Room:3-ARack:G
2023/07/10Room:3-ARack:G
2023/04/10Room:3-ARack:G
2023/02/13Room:3-ARack:G
2023/01/10Room:3-ARack:G
2022/10/11Room:3-ARack:G
2022/07/11Room:3-ARack:G

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Eno2enolase 2, gamma neuronal6Eno2<tm1(DTA)Hsak>targeted mutation 1, Hitoshi Sakno

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
DTAdiphtheria toxin A (DT-A)6DTA

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Iresinternal ribosomal entry site (EMCV)6

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)6mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

Donor DNAEncephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), phage P1 loxP sites, mouse phosphoglycerate kinase promoter (Pgk promoter), E.coli neo resistance gene, C.diphtheriae diphtheria toxin A fagment, mouse Eno2 genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Front. Behav. Neurosci., 7:17 (2013).
DepositorShigeyoshi Itohara (RIKEN)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data