Strain Data Sheet

RBRC10743

Strain Information

Image
BRC No.RBRC10743
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(Mapt-neo,-YC2.60)#Ito
Former Common nametLSL-YC2.60
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development理化学研究所　脳神経科学研究センター・糸原重美らにより開発された。C57BL/6J(日本クレア)により維持。C57BL/6J由来BAC clone ID：RP23-344E9
Strain descriptionTauプロモーターの制御下においてCre/loxPシステムでYCレポーター（YC2.60）を発現する系統。
Colony maintenance
ReferencesCell Rep., 22(11):2873-2885 (2018). 29539417

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Actbchicken beta-actin polyAUNActb

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
WPREWoodchuck hepatitis virus posy-transcriptional regulatory element (WPRE)UNWPRE

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
YC2.60Yellow Cameleon 2.60 (Aequorea victoria ECFP cDNA, Xenopus laevis calm2 cDNA, calmodulin (CaM)-binding peptide (rabbit skeletal muscle myosin light chain kinase (MLCK) cDNA), circularly permuted form of the Venus yellow fluorescent protein cDNA)UNYC2.60

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPUNloxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)UNmouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 Intron polyAUN

Ordering Information

供与核酸mouse Tau promoter, Phage P1 loxP sites, mouse pgk promoter, E. coli neomycin resistance gene, Yellow Cameleon 2.60 (Aequorea victoria ECFP cDNA, Xenopus laevis calm2 cDNA, calmodulin (CaM)-binding peptide (rabbit skeletal muscle myosin light chain kinase (MLCK) cDNA), circularly permuted form of the Venus yellow fluorescent protein cDNA), Woodchuck hepatitis virus posttranscriptional regulatory element (WPRE), SV40 intron polyA, chicken polyA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
提供条件条件を付加する。
研究成果の公表にあたって寄託者の指定する文献を引用する。Cell Rep., 22(11):2873-2885 (2018).
研究成果の公表にあたってVenusの開発者である宮脇敦史先生への謝辞の表明並びに指定する文献を引用する。Nat. Biotechnol., 20, 87-90 (2002).
Depositor糸原　重美（国立研究開発法人理化学研究所）
Strain Status凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
凍結胚を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER of the YC2.60 using Approval form (Form V (English)) (Lab Contact: Laboratory for Cell Function Dynamics, RIKEN CBS: mta-cfds@ml.riken.jp).
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-
Sequence of Y2.60

BRC mice in Publications

No Data