Strain Information | |
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Image | |
BRC No. | RBRC10743 |
Type | Transgene |
Species | Mus musculus |
Strain name | C57BL/6J-Tg(Mapt-neo,-YC2.60)#Ito |
Former Common name | tLSL-YC2.60 |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed by Shigeyoshi Itohara at RIKEN. A BAC transgenic DNA construct was injected to C57BL/6J. C57BL/6J genetic background. |
Strain description | Transgenic mice expressing YC2.60 under the control of Tau promoter and Cre recombinase. |
Colony maintenance | |
References | Cell Rep., 22(11):2873-2885 (2018). 29539417 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Actbchicken beta-actin polyAUNActb Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter WPREWoodchuck hepatitis virus posy-transcriptional regulatory element (WPRE)UNWPRE Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter YC2.60Yellow Cameleon 2.60 (Aequorea victoria ECFP cDNA, Xenopus laevis calm2 cDNA, calmodulin (CaM)-binding peptide (rabbit skeletal muscle myosin light chain kinase (MLCK) cDNA), circularly permuted form of the Venus yellow fluorescent protein cDNA)UNYC2.60 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxPUNloxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)UNmouse phosphoglycerate kinase promoter (PGK promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 Intron polyAUN |
Ordering Information | |
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Donor DNA | mouse Tau promoter, Phage P1 loxP sites, mouse pgk promoter, E. coli neomycin resistance gene, Yellow Cameleon 2.60 (Aequorea victoria ECFP cDNA, Xenopus laevis calm2 cDNA, calmodulin (CaM)-binding peptide (rabbit skeletal muscle myosin light chain kinase (MLCK) cDNA), circularly permuted form of the Venus yellow fluorescent protein cDNA), Woodchuck hepatitis virus posttranscriptional regulatory element (WPRE), SV40 intron polyA, chicken polyA |
Research application | Cre/loxP system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cell Rep., 22(11):2873-2885 (2018).In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Venus, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested. Nat. Biotechnol., 20, 87-90 (2002). |
Depositor | Shigeyoshi Itohara (RIKEN) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Sequence of Y2.60 |
BRC mice in Publications |
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No Data |